mouse anti inos (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology mouse anti inos
Mouse Anti Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2638 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+inos/NOS2+Antibody/pmc13017736-122-18-21
Average 96 stars, based on 2638 article reviews

mouse anti inos - by Bioz Stars, 2026-11

96/100 stars

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Article Title: Enhanced Targeted Repair of Vascular Injury by Apoptotic‐Cell‐Mimicking Nanovesicles Engineered with P‐Selectin Binding Peptide
Article Snippet: Modulating inflammation is crucial for repairing vascular injury.. Phagocytosis of apoptotic cells represents an effective mechanism for attenuating inflammation and improving regeneration during natural healing.. However, strategies for repairing vascular injuries using biomaterials derived from apoptotic cells are still undeveloped.

Immunopeptidomics:

Article Title: Systemic microbial antigen administration ameliorates experimental autoimmune encephalomyelitis via MHC-II downregulation in the CNS and secondary lymphoid organs.
Article Snippet: .. The antibodies used were polyclonal rabbit anti-Iba-1 (019-19741, Wako, 1:1000 dilution), goat anti-CD206 (sc34577, Santa Cruz, 1:200 dilution), mouse anti-iNOS (sc7271, Santa Cruz, 1:200 dilution), monoclonal rabbit antiTmem119 (ab209064, Abcam, 1:100 dilution), monoclonal rat anti-Ly6C (ab15627, Abcam, 1:50 dilution), and mouse anti- MHC-II (ab6403, Abcam, 1:50 dilution). .. Secondary antibodies were goat anti-rabbit 488A (20012, Biotium, 1:200 dilution), goat anti-mouse 555 (20030, Biotium, 1:200 dilution), donkey anti-goat 555 (20039, Biotium, 1:200 dilution), and goat anti-rat 488A (20023, Biotium, 1:200 dilution).

Article Title: Systemic microbial antigen administration ameliorates experimental autoimmune encephalomyelitis via MHC-II downregulation in the CNS and secondary lymphoid organs
Article Snippet: .. The antibodies used were polyclonal rabbit anti-Iba-1 (019-19741, Wako, 1:1000 dilution), goat anti-CD206 (sc-34577, Santa Cruz, 1:200 dilution), mouse anti-iNOS (sc-7271, Santa Cruz, 1:200 dilution), monoclonal rabbit anti-Tmem119 (ab209064, Abcam, 1:100 dilution), monoclonal rat anti-Ly6C (ab15627, Abcam, 1:50 dilution), and mouse anti- MHC-II (ab6403, Abcam, 1:50 dilution). .. Secondary antibodies were goat anti-rabbit 488A (20012, Biotium, 1:200 dilution), goat anti-mouse 555 (20030, Biotium, 1:200 dilution), donkey anti-goat 555 (20039, Biotium, 1:200 dilution), and goat anti-rat 488A (20023, Biotium, 1:200 dilution).

Western Blot:

Article Title: CCR3 knockdown attenuates prolonged underwater operations-induced cognitive impairment via alleviating microglia-mediated neuroinflammation.
Article Snippet: IHC:1:1000) Abcam Cat#ab187041; RRID: AB_2892613 .. Mouse anti-iNOS (IF:1:500) Santa Cruz Cat#sc-7271; RRID: AB_627810 Rabbit anti-GAPDH (Western blotting:1:1000) Cell Signaling Technology Cat#5174S; RRID: AB_10622025 Bacteria and virus strains pHBAAV-U6-MCS-CMV-EGFP-CCR3 shRNA Hanbio Tech N/A Chemicals, peptides, and recombinant proteins TRIzol reagent Invitrogen Cat# 15596018 Protease and phosphatase inhibitor cocktail NCM Biotech Cat# P002 RIPA lysate buffer Beyotime Cat# P0013B BCA kit Thermo Cat# 23250 Stripping buffer Service bio Cat# G2078 Critical commercial assays Human Cortisol ELISA kit Elabscience Biotechnology Cat# E-OSEL-H0006 Rat IL-1 beta ELISA Kit Servicebio Cat# GER0002 Rat IL-6 ELISA Kit Servicebio Cat# GER0001 Rat TNF-alpha ELISA Kit Servicebio Cat# GER0004 Rat Corticosterone ELISA kit Elabscience Biotechnology Cat# E-OSEL-R0002 First Strand cDNA synthesis Kit Invitrogen Cat# K1622 QuantityNova SYBR Green PCR Kit Qiagen Cat# 208054 Oligonucleotides RT-qPCR primers, see Table S1 This paper N/A shCCR3 50-CAGCTGGAAGCGTTTCCATGCTCTA-30 Hanbio Tech N/A .. Raw bulk RNAseq data This paper GSE247651 shCCR3 50-CAGCTGGAAGCGTTTCCATGCTCTA-30 Hanbio Tech N/A

Article Title: Upregulation of ACSL1 in synovial macrophages promotes lipid peroxidation via the IκB/NF-κB pathway to accelerate osteoarthritis
Article Snippet: Target bands were visualized using FDbio-Dura ECL (FDbio Science, Hangzhou, China). .. Antibodies used for western blotting were: mouse anti-iNOS (Santa Cruz Biotechnology, 1:1000, #sc-7271, USA), rabbit anti-ACSL1 (Proteintech, 1:1000, #13989-1-AP, China), rabbit anti-TNFα (Abcam, 1:1000, #ab6671, USA), rabbit anti-MMP13 (Abcam, 1:1000, #ab39012, USA), rabbit anti-p16 (Proteintech, 1:1000, #10883-1-AP, China), rabbit anti-p21 (Proteintech, 1:1000, #10355-1-AP, China), rabbit anti-SOX9 (Abcam, 1:1000, #ab185966, USA), rabbit anti-p65 (Cell Signaling Technology, 1:1000, #8242, USA), rabbit anti-IκBα (Cell Signaling Technology, 1:1000, #9242, USA), and species-matched horseradish peroxidaseconjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA). .. In this study, total RNA was extracted from primary murine bone marrow-derived macrophages (BMDM) using TRIzol reagent (Takara Bio Inc., Shiga, Japan) following standard procedures.

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Article Title: Radiologic and immunohistochemical correlates of cavitary pulmonary mycobacterial infection: A comparative study of tuberculosis and nontuberculous mycobacteria.
Article Snippet: Formalin-fixed paraffin-embedded (FFPE) lung tissue sections (4- μm thick) were prepared for immunohistochemical (IHC) analysis using a Bond-Max Automated IHC stainer (Leica Biosystems, Newcastle Ltd, Australia) according to standardized protocols. .. Sections were deparaffinized with xylene, rehydrated through graded alcohols, and subjected to heat-induced antigen retrieval: • CD68 and Arginase-1: Epitope Retrieval Solution 2 (citrate buffer, pH 9.0) at 100 ◦C for 20–30 min • iNOS: Epitope Retrieval Solution 2 (EDTA buffer, pH 9.0) at 100 ◦C for 30 min • PTMA: Epitope Retrieval Solution 1 (citrate buffer, pH 6.0) at 100 ◦C for 20 min, followed by 20-min protein blocking at room temperature Primary antibodies used: • Mouse anti-CD68 (1:100, Leica Biosystems) • Rabbit anti-Arginase-1 (1:50, ZETA Corporation) • Mouse anti-iNOS (NOS2) (1:50, Santa Cruz Biotechnology, sc-7271) • Mouse anti-PTMA (1:32, custom-made) Primary antibody incubation was performed at room temperature for 30–60 min. .. Sections were then treated with post-primary reagent (8 min), polymer detection reagent (8 min), and hydroperoxide blocking (5 min), using the BondTM Polymer Refine Detection Kit (catalog no. DS9800, Leica).

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